Pathogenicity of bacteria and viruses to Spodoptera frugiperda (Lepidoptera: Noctuidae)

Elisangela de Souza Loureiro; Lidiane Arissa Yokota; Gabriella Silva de Gregori; Andressa Silva Rodrigues; Luis Gustavo Amorim Pessoa; Pamella Mingotti Dias

doi:10.5327/Z2176-94781952

Authors

Elisangela de Souza Loureiro Federal University of Mato Grosso do Sul (UFMS) - Brazil https://orcid.org/0000-0002-9708-3775
Lidiane Arissa Yokota Federal University of Mato Grosso do Sul (UFMS) - Brazil https://orcid.org/0000-0001-5478-9930
Gabriella Silva de Gregori Federal University of Mato Grosso do Sul (UFMS) - Brazil https://orcid.org/0009-0003-8577-0986
Andressa Silva Rodrigues Federal University of Mato Grosso do Sul (UFMS) - Brazil https://orcid.org/0000-0001-5602-1474
Luis Gustavo Amorim Pessoa Federal University of Mato Grosso do Sul (UFMS) - Brazil https://orcid.org/0000-0003-4646-062X
Pamella Mingotti Dias Federal University of Grande Dourados (UFGD) - Brazil https://orcid.org/0000-0002-0963-9455

DOI:

https://doi.org/10.5327/Z2176-94781952

Keywords:

fall armyworm; integrated pest management; sustainable agriculture.

Abstract

Combining integrated pest management and biological control has been an effective, economical, and sustainable strategy for controlling agricultural pests. The larvae of Spodoptera frugiperda, apest of several crops, cause financial losses to the agribusiness sector, and entomopathogens have been widely used for the biological control of this species. Therefore, the objective of this study was to evaluate the pathogenicity of bacteria and viruses for the control of S. frugiperda under laboratory conditions. Two bioassays were conducted in a completely randomized design, one using second-instar and other using third-instar larvae, with six treatments and five replications. The entomopathogens used were S. frugiperdaMultiple nucleopolyhedrovirus (SfMNPV), Autographa californica multiple nucleopolyhedrovirus (AcMNPV), Bacillus subtilis, B. thuringiensis subsp. thoworthy, and B. thuringiensis subsp. kurstaki. Mortality was assessed daily until the end of the larval cycle. Analysis of variance (F-test) was applied, followed by mean comparison through the Scott-Knott test at a 5% significance level. The efficacy of the entomopathogens as bioinsecticides was evaluated using Abbott’s formula. Overall, the most effective entomopathogens for controlling second-instar S. frugiperdalarvae were SfMNPV and B. subtilis, resulting in 100% control, whereas AcMNPV was the most effective against third-instar larvae, showing maximum lethality in 76.67% of samples.

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